Development and Application of nanoPCR Method for Detection of Feline Panleukopenia Virus

Feline panleukopenia (FP) is a severe viral illness caused by the feline virus (FPV), putting sectors like companion cat breeding and endangered conservation at risk. The has high morbidity fatality rate found all over world. We created novel FPV assay using nanoPCR technology assessed method’s specificity sensitivity. approach amplified 345 bp nucleic acid fragment with minimum detection limit of 7.97 × 102 copies/μL, which about 100 times greater than traditional PCR. collected anal swabs from 83 cats suspected infection for practical application, FPV-positive determined was 77.1%. In conclusion, more sensitive conventional PCR convenient cost-effective qPCR methodology may be utilized clinical FPV.